Multifocal Plane Microscopy

Designing the Plane Spacing for Multifocal Plane Microscopy

Post Title

Introduction

Multifocal plane microscopy (MUM) has made it possible to study subcellular dynamics in 3D at high temporal and spatial resolution by simultaneously imaging distinct planes within the specimen. An important question in MUM experiments is how the number of focal planes and their spacings should be chosen to achieve the best possible localization accuracy along the z-axis.


MUMDesignTool

The MUMDesignTool is a new GUI based toolbox that allows users to calculate the limits of the accuracy with which the 3D location of a single molecules can be estimated when imaged using a MUM setup. The software supports the calculation of these limits along the z-axis for different focal planes in real-time and thereby helps to optimize the design of MUM experiments. The user can calculate limits for a variety of imaging scenarios.


Post Title

Download Software

For more information and to dowanload the software, please visit the MUMDesignTool homepage.

  • Software developed in MATLAB (Optimization Toolbox, Image Processing Toolbox, Compiler)
  • Based on object-oriented programming (has a graphical user interface)
  • Add-ons using Autohotkey

Related Talk


Related publications

1
SPIE
A. Tahmasbi, S. Ram, J. Chao, A. V. Abraham, E. S. Ward, and R. J. Ober, "An information-theoretic approach to designing the focal plane spacing for multifocal plane microscopy," in Proc. SPIE International Symposium on Biomedical and Optics (BiOS), 9330: 933011, San Francisco, CA, February 11, 2015.
2
QBI
A. Tahmasbi, S. Ram, J. Chao, A. V. Abraham, E. S. Ward, and R. J. Ober, "Mumdesigntool: A software module for designing the plane spacing for multifocal plane microscopy," Quantitative BioImaging 2015, Institut Pasteur, Paris, France, January 7, 2015.
3
IF 3.488
A. Tahmasbi, S. Ram, J. Chao, A. V. Abraham, F. W. Tang, E. S. Ward, and R. J. Ober, "Designing the focal plane spacing for multifocal plane microscopy," Optics Express, vol. 22, no. 14, pp. 16706-16721, 2014.

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